Agilent 1260 infinity ii инструкция на русском

Дополнительное оборудование для ВЭЖХ системы Agilent 1260 II

Вспомогательное оборудование для ВЭЖХ системы Agilent 1260 II

• 58, Agilent Technologies 1260 Infinity II 58 1260 Infinity II Multicolumn Thermostat User Manual 3 Using the Module Installing and Using Colunm ID Tags Installing and Using Colunm ID Tags Install the Column Identification Option Parts required p/n Description 5067-5915 Column ID Kit Left 5067-5917 Column ID TAG Assembly Preparations Power off the instrument. CAUTION Electronic boards and components are sensitive to electrostatic discharge (ESD). ESD can damage electronic boards and components. ➔ Be s…

• 180, 180 1260 Infinity II Multicolumn Thermostat User Manual 13 Appendix Waste Electrical and Electronic Equipment Directive Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005. NOTE This product complies with th…

• 50, 50 1260 Infinity II Multicolumn Thermostat User Manual 3 Using the Module Installing Valve Heads 1 Switch off the module. 2 When unscrewing the transportation lock, push it back until the last screw is removed — the valve rail is spring-loaded. 3 Press on the valve dummy (1.) to release it (2.) (spring-loaded valve rail). 4 5  
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• 53, Agilent Technologies 1260 Infinity II 1260 Infinity II Multicolumn Thermostat User Manual 53 Using the Module 3 Installing Valve Heads The following procedure shows the valve head installation with an G7116 (MCT) module as an example. For other modules it is similar. 1 Insert the valve head into the valve shaft. OR If the outside pin does not fit into the outside groove, you have to turn the valve head until you feel that the two pins snap into the grooves. Now you should feel additional resistance from the valve drive while continuously turning the valve head until the pin fit…

• 83, 83 1260 Infinity II Multicolumn Thermostat User Manual Agilent Technologies 5 Optimization Optimizing the Column Compartment 84 This chapter provides information on how to optimize the Multi Column Thermostat.
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• 10, 10 1260 Infinity II Multicolumn Thermostat User Manual 1 Introduction to the Multicolumn Thermostat Product Description (G7116A) Product Description (G7116A) The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) facilitates precise column thermostatting over a broad temperature range with cooling down to 10 degrees below ambient and heating up to 85 °C. This capability provides robust and reliable separations for maximum application flexibility. Exchangeable hi…

• 106, 106 1260 Infinity II Multicolumn Thermostat User Manual 8 Test Functions and Calibrations System Pressure Test System Pressure Test For running a System Pressure Test, please refer to the corresponding pump manual. The System Pressure Test may be used for testing the tightness of a valve installed in the MCT. CAUTION Wrong use of System Pressure Test may damage components. ➔ Do not use higher test max. pressure settings as the lowest rated pressure component in…

• 84, Agilent Technologies 1260 Infinity II 84 1260 Infinity II Multicolumn Thermostat User Manual 5 Optimization Optimizing the Column Compartment Optimizing the Column Compartment For best performance results of the multi column compartment: • Use short connection capillaries and place them close to the heatexchanger. This will reduce heat dissipation and external band-broadening. • See the Consumables sections for additional available heat exchangers with various internal volume to address certai…

• 1, Agilent Technologies Agilent InfinityLab LC Series 1260 Infinity II Multicolumn Thermostat User Manual
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• 11, 1260 Infinity II Multicolumn Thermostat User Manual 11 Introduction to the Multicolumn Thermostat 1 Features (G7116A) Features (G7116A) • Superior usability with flexible flap positions: open door to 90° (desk function), or to 180° or even removal of the front door for maximum accessibility. • Efficient, fast, and most convenient column exchange through InfinityLab Quick Connect fittings. • Reproducible precolumn solvent thermostatting with next generation InfinityLab Quick…

• 80, 80 1260 Infinity II Multicolumn Thermostat User Manual 4 Preparing the Module Solvent Information organic solvents used in chromatographic systems like methanol, acetonitrile and isopropanol. It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar. Tantalum (Ta) Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids w…

• 179, Agilent Technologies 1260 Infinity II 1260 Infinity II Multicolumn Thermostat User Manual 179 Appendix 13 General Safety Information CAUTION General hazards and improper disposal Improper disposal of the media and components used pollutes the environment. ➔ The breakdown of the sample cooler unit must be carried out by specialist refrigeration company. ➔ All media must be disposed of in accordance with national and local regulations. ➔ Please contact your local Agilent Service Center in regard to safe environmental …

• 163, 1260 Infinity II Multicolumn Thermostat User Manual 163 Hardware Information 12 Interfaces It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements. The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique). To provide maximum safety within a distributed analysis system, one line is…

• 162, 162 1260 Infinity II Multicolumn Thermostat User Manual 12 Hardware Information Interfaces Overview Interfaces CAN The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. LAN The modules have either an interface slot for an LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This i…

• 166, 166 1260 Infinity II Multicolumn Thermostat User Manual 12 Hardware Information Interfaces IO (Input/Output) Lines • Eight generic bi-directional channels (input or output). • Same as the APG Remote. • Devices like valves, relays, ADCs, DACs, controllers can be supported/controlled. 1-Wire Data (Future Use) This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type…

• 14, 14 1260 Infinity II Multicolumn Thermostat User Manual 1 Introduction to the Multicolumn Thermostat Column Switching Valve Column Switching Valve InfinityLab QuickChanges Valves for column switching are an orderable add-on option for the InfinityLab Multicolumn Thermostat. Multicolumn Selection (G7116A with G4237A) Advantages • Increase productivity • Higher instrument up-time The valve facilitating quick changes…

• 93, Agilent Technologies 1260 Infinity II 1260 Infinity II Multicolumn Thermostat User Manual 93 Error Information 7 General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak senso…

• 82, 82 1260 Infinity II Multicolumn Thermostat User Manual 4 Preparing the Module Solvent Information Platinum/Iridium Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, a…

• 69, 1260 Infinity II Multicolumn Thermostat User Manual 69 Using the Module 3 Installing and Using Colunm ID Tags Figure 14 The Dashboard panel of the column compartment Place the mouse cursor over the column ID tag icon ( ); the tooltip shows the information currently stored on the column ID tag. Note that the column ID tag icon changes according to its state as described in “The Column Tag Information Table” on page 71.
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• 28, Agilent Technologies 1260 Infinity II 28 1260 Infinity II Multicolumn Thermostat User Manual 2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 31. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. W…

• 169, 1260 Infinity II Multicolumn Thermostat User Manual 169 Hardware Information 12 Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed. This pack is th…

• 37, Agilent Technologies 1260 Infinity II 37 1260 Infinity II Multicolumn Thermostat User Manual Agilent Technologies 3 Using the Module Magnets 38 Turn on/off 39 Status Indicators 41 Open the Front Door 42 Install Heat Exchanger 43 Exchange a Column 46 Installing Valve Heads 49 Remove the Transportation Lock and the Valve Dummy 49 Install the Valve Head and Connect Capillaries 51 Installing the capillaries 55 Install the Divider Assembly 57 Installing and Using Colunm ID Tags 58 Install the Column Identification Option 58 Connecting the Column ID Tags 61 Usin…

• Agilent Technologies 1260 Infinity II 194 Agilent 1260 Infinity II Preparative Autosampler User Manual 10 Parts and Materials for Maintenance External Tray External Tray Figure 25 External tray p/n Description G7129-60000 External Tray for 5 x 2 mL Vials G1313-27302 Disposal tube
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 263 Appendix 14 Refrigerant WARNING Refrigerant Refrigerant HFC-134a is known as a safe refrigerant, however accidents can occur if it is handled incorrectly. For this reason, the following instructions must be observed: ➔ Avoid contact with liquid refrigerant HFC-134a. At atmospheric pressure HFC-134a evaporates at approximately -26 °C and causes frost bite. ➔ After skin contact, rinse the affected area with water. ➔ After eye contact, rinse the eye(s) with plenty of water for at least 15 minutes an

• Agilent Technologies 1260 Infinity II 130 Agilent 1260 Infinity II Preparative Autosampler User Manual 8 Error Information General Error Messages General Error Messages Timeout Error ID: 0062 The timeout threshold was exceeded. Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message. Probable cause Suggested actions 1 The analysis was completed successfully, and the timeout function switched off the mod

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 121 Troubleshooting and Diagnostics 7 Troubleshooting Troubleshooting If the autosampler is unable to perform a specific step due to a hardware failure, an error message is generated. You can use the injector steps to do the injection sequence, while observing how the instrument responds. “Automatic Referencing” on page 117 summarizes the injector steps, and lists the associated error messages and probable causes of step failures.
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 259 Appendix 14 General Safety Information Symbols Table 2 2 Symbols The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages. Indicates a protected ground terminal. The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up. Cooling unit is designed as vapor-compression refrigeration syste

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 69 Installation of the Optional Sample Cooler 4 Using the Sample Cooler in an Infinity II Sampler Online Signal Monitor The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals. The Online Plot Window should look like this: If the change button is pressed you can select the Chiller signal WPS1A in the online plot.
  

• 250 Agilent 1260 Infinity II Preparative Autosampler User Manual 13 LAN Configuration PC and Agilent ChemStation Setup 12 The left column shows the modules that could be configured. You may select the module manually from the list. We use the Auto Configuration mode. Click on Yes. 13 Enter the IP address or the Hostname of the module with the LAN-access. 14 Click on OK. The selected module is shown now in the right window (with serial number). In addition all other modules connected via

• Agilent 1260 Infinity II Preparative Autosampler User Manual 29 Site Requirements and Specifications 2 Physical Specifications Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 19 kg (41.9 lbs) w/o sample cooler Dimensions (height × width × depth) 320 x 396 x 468 mm (12.8 x 15.6 x 18.4 inches) Line voltage 100 – 240 V~, ± 10 % Wide-ranging capability Line frequency 50 or 60 Hz, ± 5 % Power consumption 350 VA / 350 W / 1195 BTU/h Ambient op

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 87 Preparing the Module 5 Setting up the Sampler Setting up the Sampler The setup of the sampler is shown exemplarily for the Vialsampler. Table 7 The Vialsampler User Interface Within the Vialsampler user interface, there are active areas. If you move the mouse cursor across the icons (tray, EMF button), the cursor will change and you may click on the icon to 1 Turn on /off the Vi als ampler 2 Check which sample drawers are installed 3 Get the status of the

• 148 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. Status indicator Needle assembly Drawer Column shelf Leak drain Power switch NOTE There are no serviceable parts inside. Do not open the module.
  

• Agilent Technologies 1260 Infinity II 174 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Metering Seal and Piston 3 Disconnect the two capillaries from the metering device. 4 Remove the two fixing bolts. 5 Pull the metering head assembly away from the sampler. 6 Remove the two fixing bolts from the base of the metering head assembly. 7 Disassemble the metering head assembly. 8 Use a seal insert tool to carefully remove the seal.
  

• 192 Agilent 1260 Infinity II Preparative Autosampler User Manual 10 Parts and Materials for Maintenance Drawer Assembly Drawer Assembly Figure 22 Drawer for 66 x 2.0 mL Vials Figure 23 Drawer for 18 x 6 mL Vials Item p/n Description G7129-60010 Drawer for 66 x 2 mL Vials G7129-60110 Drawer for 18 x 6 mL Vials G7129-68210 Drawer-Kit 100 x 2 mL Vials Classic 1 G7129-60210 Drawer for 50 x 2 mL Vials Classic Left 2 G7129-60220 Drawer for 50 x 2 mL Vials Classic Right G7129-68210 Drawer-

• 124 Agilent 1260 Infinity II Preparative Autosampler User Manual 7 Troubleshooting and Diagnostics Troubleshooting Guide for the Sample Transport Assembly Poor alignment With Error messages • motor overtemp (0 or 2 or 3) • movement failed (0 or 2 or 3) 1 Start automatic referencing in Lab Advisor. For this procedure it is mandatory to use the calibration tool. On completion of the procedure, the corrected values are stored in the instrument firmware. 2 Exchange the gripper arm

• Agilent 1260 Infinity II Preparative Autosampler User Manual 179 Maintenance 9 Replace the Peristaltic Pump Cartridge Replace the Peristaltic Pump Cartridge When Tubing blocked or broken Tools required Description Parts required p/n Description 5065-4445 Peristaltic pump with Pharmed tubing Preparations Remove the inlet filter of the solvent bottle which guides the solvent to the peristaltic pump to avoid syphoning effects. WARNING When opening capillary or tube fittings solvents may leak out. The handling of toxic and hazardous solvents and reagents can hold health risks. ➔ Please observ

• Agilent Technologies 1260 Infinity II 60 Agilent 1260 Infinity II Preparative Autosampler User Manual 4 Installation of the Optional Sample Cooler Install the Sample Cooler Install the Sample Cooler Parts required p/n Description Sampler G7167-60005 Sample cooler Power cord 5067-6208 Condensate Drainage Kit G7167-90170 Technical Note — Installation of the Infinity II Cooler Condensate Drainage Tubing Kit Preparations Sampler is installed in the stack. CAUTION Condensate inside the sample cooler Damage to the electronics of the module ➔ Before dismounting th

• Agilent Technologies 1260 Infinity II 26 Agilent 1260 Infinity II Preparative Autosampler User Manual 2 Site Requirements and Specifications Site Requirements Site Requirements Site Requirements A suitable environment is important to ensure optimum performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 29. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic e

• 260 Agilent 1260 Infinity II Preparative Autosampler User Manual 14 Appendix General Safety Information Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields. Indicates a pinching or crus

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 269 Index firmware description 212 main system 212 resident system 212 update tool 213 updates 213, 187 upgrade/downgrade 187 flow connections 85 frequency range 29 G gripper alignment 117 gripper fingers 21 gripper alignment 109 external vials 109 guide coding 82 color 82 H humidity 29 hydraulic box 19 I ICC leak sensor 22, 22 injection sequence 14 injection valve 19, 20 injection volume precision 104 injection volumes less than 2 µl 104 injection volumes 104 installation bench space 28 sample cooler 60 install doors 153 flow connections 85 instrument layout 229 interfaces I

• 152 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path.
  

• Agilent Technologies 1260 Infinity II 240 Agilent 1260 Infinity II Preparative Autosampler User Manual 13 LAN Configuration Manual Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it. Figure 38 Manual Configuration (Principle) 7(/1(7 6HVVLRQ &

• Agilent Technologies 1260 Infinity II Agilent Technologies Agilent InfinityLab LC Series 1260 Infinity II Preparative Autosampler User Manual
  

• 102 Agilent 1260 Infinity II Preparative Autosampler User Manual 6 Optimizing Performance Fast Injection Cycle and Low Delay Volume Fast Injection Cycle and Low Delay Volume Short injection cycle times for high sample throughput is one of the most important requirements in laboratories. In order to shorten cycle times, you can: • shorten the column length • use high flow rates • apply a steep gradient Having optimized these parameters, further reduction of cycle times can be

• Agilent Technologies 1260 Infinity II 54 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Agilent Local Control Modules Agilent Local Control Modules Agilent InfinityLab LC Companion G7108AA The Agilent InfinityLab LC Companion gives you complete control, system monitoring, signal plotting and diagnostic capabilities for a wide range of LC system modules. The instrument control solution is available as full package including all hardware and accessories, but can also be used on your own mobile devices like tablets, mobile phones and other electronic equipment. Combining the conveniences of the Agilent Instant Pilot fea

• Agilent Technologies 1260 Infinity II 226 Agilent 1260 Infinity II Preparative Autosampler User Manual 12 Hardware Information Setting the 6-bit Configuration Switch Table 1 5 6-bit Configuration Switch Mode Function/Setting Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 COM 1 1 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from non-volatile memory. 0 n.a. 2 2 not assigned — Always keep these switches on position ‘0’ (off) n.a. LAN Init Mode n.a. Use Default IP Address 3 3 Default IP Address is 192.168.254.11 00000 Use Stored IP Address 0 0 0 1 0 Use DHCP to request IP Address

• Agilent Technologies 1260 Infinity II 114 Agilent 1260 Infinity II Preparative Autosampler User Manual 7 Troubleshooting and Diagnostics Maintenance Functions Park Arm User Interface In the control software the Park Arm command is part of the ALS positions that can be selected from the special commands menu in the Instrument Control display. The commands for the control software are: Park Arm moves the gripper arm to the park position. Home moves the gripper arm out of the park position to the home position.
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual Notices © Agilent Technologies, Inc. 2017 No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla- tion into a foreign language) without prior agreement and written consent from Agi- lent Technologies, Inc. as governed by United States and international copyright laws. Manual Part Number G7157-90000 Edition 09/2017 Printed in Germany Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn Warranty The material contained in this docu- ment is provided “as is,” and is sub- ject t

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 145 Error Information 8 Sample Cooler Error Messages Cooler temperature below or above target temperatur. Error ID: 30705, 30706 The sample cooler temperature is above or below the target temperature. Cooler Overpressure Failure Error ID: 30712 The sample cooler pressure sensor has detected an overpressure. Probable cause Suggested actions 1 Condensate inside of the chiller Check if the drainage is ok. 2 Defective cable Check the signal cable from the ALS to the sample cooler. 3 Defective temperature sensor Replace the sample cooler. Probable cause Suggest

• Agilent 1260 Infinity II Preparative Autosampler User Manual 89 Preparing the Module 5 Setting up the Sampler Figure 17 ChemStation Method and Run Control
  

• Agilent Technologies 1260 Infinity II 242 Agilent 1260 Infinity II Preparative Autosampler User Manual 13 LAN Configuration Manual Configuration 4 To change a parameter follows the style: • parameter value, for example: ip 134.40.28.56 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. 5 Use the “/” and press Enter to list the current settings. Table 2 1 Telnet Commands Value Description ? displays syntax and descriptions of command

• Agilent 1260 Infinity II Preparative Autosampler User Manual 245 LAN Configuration 13 PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration.
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 109 Troubleshooting and Diagnostics 7 Overview of the Module’s Indicators and Test Functions Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually. The step functions are used primarily for troubleshooting, and for verification of correct autosampler operation after repair (see “Step Commands” on page 119).
  

• 182 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Wash Port Assembly Exchange the Wash Port Assembly When Defective flapper Parts required p/n Description G7129-60033 Saftey wash port (needle station) Preparations Finish any pending acquisition job and in order to avoid leaks, stop the pump running and remove the tubings from the solvent bottles. If available close the shutoff valves. 1 In the Local Controller start the maintenance mode and select Change needle/seat function. OR In the Agil

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 51 Using the Module 3 Choice of Vials and Caps Screw Caps p/n Description 5182-0717 Screw Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0718 Screw Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0719 Screw Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0720 Screw Cap, blue polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0721 Screw Cap, green polypropylene, septum (clear PT

• Agilent 1260 Infinity II Preparative Autosampler User Manual 13 Introduction 1 Operating Principle Operating Principle Sampling Sequence The preparative autosampler processor continuously monitors the movements of the preparative autosampler components during the sampling sequence. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence can’t be completed successfully, an error message is generated. During the sampling sequence, the solvent bypasses the preparative sampler via the injection valve.

• 8 Agilent 1260 Infinity II Preparative Autosampler User Manual Contents 12 Hardware Information 211 Firmware Description 212 Electrical Connections 215 Interfaces 217 Setting the 6-bit Configuration Switch 225 Instrument Layout 229 Early Maintenance Feedback (EMF) 230 13 LAN Configuration 231 What You Have to Do First 232 TCP/IP parameter configuration 233 Configuration Switches 234 Initialization Mode Selection 235 Dynamic Host Configuration Protocol (DHCP) 237 Manual Configu

• Agilent 1260 Infinity II Preparative Autosampler User Manual 203 Identifying Cables 11 Analog Cables Agilent Module to BNC Connector Agilent Module to General Purpose p/n 8120-1840 Pin BNC Pin Agilent module Signal Name Shield Shield Analog — Center Center Analog + p/n 01046-60105 Pin Pin Agilent module Signal Name 1Not connected 2 Black Analog — 3 Red Analog +
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 71 Installation of the Optional Sample Cooler 4 Using the Sample Cooler in an Infinity II Sampler Important Information • If the temperature is too warm or too cold in the chiller, check the air vents first to make sure they are not blocked. • If frost and ice build up inside the sample cooler, defrost the chiller. This is best done overnight. After defrosting, first check the drainages of the sample cooler to make sure they are not blocked. • Waiting for the autosampler to cool down can take 30 min — 45 min or more. This slow ramping behavior is necessary to a

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 165 Maintenance 9 Exchange the Sample Loop Assembly 3 Unscrew the safety cover screw. WARNING Sharp needle Uncovered needles may cause injuries ➔ Do not touch the tip of the needle. 4 Remove the safety cover. 5 Check if the needle is in maintenance position. 6 Disconnect the loop capillary from the metering device and from the needle.
  

• Agilent Technologies 1260 Infinity II 94 Agilent 1260 Infinity II Preparative Autosampler User Manual 5 Preparing the Module Setting up the Sampler High Throughput Injection Valve to Bypass for Delay Volume Reduction: Mark this check box to switch the flow from the injector from mainpass to bypass after injection has taken place. This reduces the delay volume for low volume techniques. You can specify the point during the analyses when the valve switches to bypass by setting the Sample Flush-out Factor. Sample Flush-Out Factor: ensures that the sample is thoroughly flushe

• 222 Agilent 1260 Infinity II Preparative Autosampler User Manual 12 Hardware Information Interfaces ERI Description The ERI interface contains eight individual programmable input/output pins. In addition, it provides 24 V power and 5 V power and a serial data line to detect and recognize further add-ons that could be connected to this interface. This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 29

• Agilent 1260 Infinity II Preparative Autosampler User Manual 261 Appendix 14 Waste Electrical and Electronic Equipment Directive Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005. NOTE This product complies with the WEEE Directive (2002/96/EC) marking require

• Agilent Technologies 1260 Infinity II 56 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Agilent Local Control Modules
  

• Agilent Technologies 1260 Infinity II 154 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Needle Assembly Exchange the Needle Assembly When When the limit in the needle into seat counter in the EMF is exceeded or when needle shows indications of damage, blockage or leaks. Tools required p/n Description 8710-1924 Wrench open 14 mm 8710-2140 Screwdriver Torx TX-10 Pair of pliers Parts required p/n Description G7157-87201 Needle High Flow Infinity II Autosampler Preparations In order to avoid leaks, stop the pump running and remove the tubings from the solvent bottles. If

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 81 Preparing the Module 5 Solvent Information TFE/PDD copolymer tubings, which are used in all Agilent degassers except 1322A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of Hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in

• 230 Agilent 1260 Infinity II Preparative Autosampler User Manual 12 Hardware Information Early Maintenance Feedback (EMF) Early Maintenance Feedback (EMF) Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval. The early maintenance feedback (EMF) feature monitors the usage of specific components in the instrument, and provid

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 187 Maintenance 9 Replace the Module Firmware Replace the Module Firmware To upgrade/downgrade the module’s firmware carry out the following steps: 1 Download the required module firmware, the latest FW Update Tool and the documentation from the Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. When The install

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 247 LAN Configuration 13 PC and Agilent ChemStation Setup Agilent ChemStation Setup This procedure describes the Agilent ChemStation B.04.02 setup for the 1290 Infinity system using the 1290 Infinity DAD (G4212A) as the interfacing module. The setup works in the same way for all other systems. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure — Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. NOTE The LAN must be connected to detector due to high data load on communica

• 204 Agilent 1260 Infinity II Preparative Autosampler User Manual 11 Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male — open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male — male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 pin Color code Enhanced Remote Classic Remote Active (TTL) 1white IO1 START REQUEST Low 2brown IO2 STOP Low 3 green IO3 READY High 4yellow

• Agilent 1260 Infinity II Preparative Autosampler User Manual 257 Appendix 14 General Safety Information In Case of Damage WARNING Damage to the module Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel.
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 101 Optimizing Performance 6 Optimization for Lowest Carry-over General Recommendation to Lowest Carry-Over For samples where the outside of the needle cannot be cleaned sufficiently with the wash port use wash vials with an appropriate and stronger solvent. An injector program and an additional wash vial can be used for cleaning. In case the needle seat has got contaminated and carry-over is significantly higher than expected, the following procedure can be used to clean the needle seat: • In

• Agilent Technologies 1260 Infinity II 98 Agilent 1260 Infinity II Preparative Autosampler User Manual 6 Optimizing Performance Optimization for Lowest Carry-over Optimization for Lowest Carry-over Several parts of an injection system can contribute to carry-over: • needle outside • needle inside • needle seat • sample loop • seat capillary • injection valve The autosampler continuous flow-through design ensures that sample loop, needle inside, seat capillary, and the mainpass of the injection valve is always in the flow line. These parts are continuously flushed during an isocrat

• Agilent Technologies 1260 Infinity II 90 Agilent 1260 Infinity II Preparative Autosampler User Manual 5 Preparing the Module Setting up the Sampler Control Settings The control settings are available via right click on the active area of the grafical user interface, see Table 7 on page 87. Table 8 Control settings Figure 18 Control settings The Vialsampler control parameters are in six sections: • Missing Vial Mark the Ignore missing vial check box to specify that, if a vial is missing, the injector ignores it and continues with a 6-second dummy run. The message «Missing vial <x>» is logged, and the system continues with the next injection

• Agilent 1260 Infinity II Preparative Autosampler User Manual 129 Error Information 8 What are Error Messages What are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of th

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 83 Preparing the Module 5 Swage Fittings Swage Fittings 1 Select a nut that is long enough for the fitting you’ll be using. 2 Slide the nut over the end of the tubing. 3 Carefully slide the ferrule components on after the nut and then finger-tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting. 4 Use a column or injection valve to gently tighten the fitting which forces the ferrule to seat onto the tubing. NOTE Don’t overtighten. Overtightening will shorten the lifetime of the fitting.
  

• Agilent Technologies 1260 Infinity II 35 Agilent 1260 Infinity II Preparative Autosampler User Manual Agilent Technologies 3 Using the Module Magnets 36 Turn on/off 37 Status Indicators 39 Exchange Drawers 40 Install the External Tray 42 Sample Trays 44 Choice of Vials and Caps 48 Transporting the Sampler with a Sample Cooler Installed 52 Agilent Local Control Modules 54 This chapter provides information on how to use the module.
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 119 Troubleshooting and Diagnostics 7 Step Commands Step Commands Each movement of the sampling sequence can be done under manual control. This is useful during troubleshooting, where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair. Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions, enabling the specific step to be done. Table 1 0 Injector step commands Step Action Comments Valve Bypas

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 229 Hardware Information 12 Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed. This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet. The advantages of this packaging technology are

• 150 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Warnings and Cautions WARNING Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice. ➔

• 73 Agilent 1260 Infinity II Preparative Autosampler User Manual Agilent Technologies 5 Preparing the Module Leak and Waste Handling 74 Preparing the Module 75 Solvent Information 76 Capillary Color Coding Guide 82 Swage Fittings 83 Flow Connections to the sampler 85 Setting up the Sampler 87 Control Settings 90 Method Parameter Settings 91 Injector Programm 95 Module Configuration View 96 This chapter explains the operational parameters of the module.
  

• 132 Agilent 1260 Infinity II Preparative Autosampler User Manual 8 Error Information General Error Messages Leak Sensor Error ID: 0064 Leak A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak sen

• Agilent Technologies 1260 Infinity II 140 Agilent 1260 Infinity II Preparative Autosampler User Manual 8 Error Information Sampler Error Messages Motor Temperature Error ID: 34307 One of the motors of the transport assembly has drawn excessive current, causing the motor to become too hot. The processor has switched OFF the motor to prevent damage to the motor. See figure Figure 8 on page 21 for motor identification. • Motor 0 temperature: X-axis motor. Motor 1 temperature: Z-axis motor. Motor 2 temperature: Theta (gripper rotation) motor. Motor 3 temperature

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 19 Introduction 1 Operating Principle Hydraulic Box The hydraulic box comprises two main assemblies: metering device, and injection valve. Figure 7 Hydraulic Unit Analytical Head The analytical head is driven by the stepper motor that is connected to the drive shaft by a toothed belt. The drive nut on the spindle converts the circular movement of the spindle to linear motion. The drive nut pushes the sapphire piston against the tension of the spring into the analytical head. The base of the piston sits on the large bearing of the drive nut, which ensures the piston

• Agilent Technologies 1260 Infinity II 78 Agilent 1260 Infinity II Preparative Autosampler User Manual 5 Preparing the Module Solvent Information Polyimide Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent. Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nu

• Agilent Technologies 1260 Infinity II 122 Agilent 1260 Infinity II Preparative Autosampler User Manual 7 Troubleshooting and Diagnostics Troubleshooting Guide for the Sample Transport Assembly Troubleshooting Guide for the Sample Transport Assembly This troubleshooting guide is meant to help you diagnose and repair autosampler problems. In general, autosampler problems can be divided into three categories. 1 Intermittent lock-ups with or without vial in the gripper fingers with error messages Many times the sampler is being used very heavily. • motor overtemp (0 or 1 or 2 or 3) • movement failed (0 or 1 or 2 or 3) • missing vial 2 J

• Agilent Technologies 1260 Infinity II 14 Agilent 1260 Infinity II Preparative Autosampler User Manual 1 Introduction Operating Principle 11 The gripper arm moves out of the needle station and the wash port snaps back in position. 12 The gripper arm replaces the vial, and returns to the home position. Simultaneously, the needle lowers into the seat. 13 The injection valve switches to the mainpass position. Injection Sequence Before the start of the injection sequence, and during a preparative run, the injection valve is in the mainpass position (see Figure 2 on page 14). In this position, the mobile phase flows through the autosamplers metering device, sample l

• 100 Agilent 1260 Infinity II Preparative Autosampler User Manual 6 Optimizing Performance Optimization for Lowest Carry-over Using an Injector Program The process is based on a program that switches the bypass grove of the injection valve into the flow line for cleaning. This switching event is performed at the end of the equilibration time to ensure that the bypass grove is filled with the start concentration of the mobile phase. Otherwise the separation could be influenced, especially if microbore columns are used. Example Ou

• 24 Agilent 1260 Infinity II Preparative Autosampler User Manual 1 Introduction Leak and Waste Handling
  

• 50 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Choice of Vials and Caps Crimp Caps Snap Caps p/n Description 5181-1210 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 100/Pack 5183-4498 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 1000/Pack 5181-1215 Crimp Cap, blue aluminum, septum (clear PTFE/red rubber), 100/Pack 5181-1216 Crimp Cap, green aluminum, septum (clear PTFE/red rubber), 100/Pack 5181

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 47 Using the Module 3 Sample Trays Figure 14 Numbering of tray position (External tray)       :DVWH’LVSRVDO
  

• Agilent 1260 Infinity II Preparative Autosampler User Manual 123 Troubleshooting and Diagnostics 7 Troubleshooting Guide for the Sample Transport Assembly Intermittent lock-ups with or without vial in the gripper fingers With error messages • motor overtemp (0 or 1 or 2 or 3) • movement failed (0 or 1 or 2 or 3) •missing vial 1 Check the vials and the caps. For reliable operation, vials used with the autosampler must not have tapered shoulders or caps that are wider than the body of the vial. For

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 63 Installation of the Optional Sample Cooler 4 Install the Sample Cooler CAUTION Damage to the cables ➔ Do not bend or pinch the cables. ➔ Fit in the sample cooler perfectly. 7 Slide in the whole unit. 8 Tighten the 4 screws which holds the sample cooler unit in place. 9 Use a bubble level to check the leveling of the sampler. NOTE The sample cooler should be operated in a proper horizontal position. 10 PP
  

• Agilent Technologies 1260 Infinity II 190 Agilent 1260 Infinity II Preparative Autosampler User Manual 10 Parts and Materials for Maintenance Main Assemblies Main Assemblies Figure 21 Main assemblies Item p/n Description 1 G7129-60010 Drawer for 66 x 2 mL Vials OR 1 G7129-60110 Drawer for 18 x 6 mL Vials OR 1 G7129-68210 Drawer-Kit 100 x 2 mL Vials Classic G7129-60210 Drawer for 50 x 2 mL Vials Classic Left G7129-60220 Drawer for 50 x 2 mL Vials Classic Right 2 5067-4277 2pos/6port Injection Valve, prep, 600 bar 3 G1313-60010 Gripper assembly 4 G7129-60083 Analytical Head Assembly 900 µL 5 5065-4445 Peristalt

• Agilent 1260 Infinity II Preparative Autosampler User Manual 235 LAN Configuration 13 Initialization Mode Selection Initialization Mode Selection The following initialization (init) modes are selectable: Default IP address for LAN is 192.168.254.11. DHCP address is the module’s LAN MAC address. Using Stored When initialization mode Using Stored is selected, the parameters are taken from the non-volatile memory of the module. The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the descri

• Agilent Technologies 1260 Infinity II 206 Agilent 1260 Infinity II Preparative Autosampler User Manual 11 Identifying Cables Remote Cables • 5188-8057 ERI to APG and RJ45 (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG), Connector plug Cat5e (RJ45)) One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Table 1 2 5188-8057 ERI to APG and RJ45 p/n 5188-8057 Pin (ERI) Signal Pin (APG

• Agilent 1260 Infinity II Preparative Autosampler User Manual 149 Maintenance 9 Warnings and Cautions Warnings and Cautions WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations. ➔ Use your Agilent products only in the manner described in the Agilent product us

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 91 Preparing the Module 5 Setting up the Sampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface. Figure 19 Method parameter settings
  

• 106 Agilent 1260 Infinity II Preparative Autosampler User Manual 6 Optimizing Performance Choice of Rotor Seal Choice of Rotor Seal Vespel™ Seal (for 1290 injection valves only) The 1290 seal has sealing material made of Vespel. Vespel is suitable for applications using mobile phases within the pH range of 2.3 – 9.5, which is suitable for the majority of applications. However, for applications using mobile phases with pH below 2.3 or above 9.5, the Vesp

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 49 Using the Module 3 Choice of Vials and Caps Snap Top Vials Screw Top Vials p/n Description 5182-0544 Snap Top Vial, 2 mL, clear glass, 100/Pack 5183-4504 Snap Top Vial, 2 mL, clear glass, 1000/Pack 5183-4507 Snap Top Vial, 2 mL, clear glass, 100/Pack (silanized) 5182-0546 Snap Top Vial, 2 mL, clear glass, write-on spot, 100/Pack 5183-4505 Snap Top Vial, 2 mL, clear glass, write-on spot, 1000/Pack 5183-4508 Snap Top Vial, 2 mL, clear glass, write-on spot, 100/Pack (silanized) 5182-0545 Snap Top Vial, 2 m

• Agilent 1260 Infinity II Preparative Autosampler User Manual 163 Maintenance 9 Exchange the Needle Seat Assembly 17 Turn or flap the wash port to the right. 18 Install the safety cover (1) and fix the screw (2). 19 Select Next to move the needle into the needle seat. 20 Select Back to finish the procedure. 21 Close the doors. 22 Perform a pressure test. 1 2
  

• Agilent Technologies 1260 Infinity II 176 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Metering Seal and Piston 15 Reinstall the capillaries. 16 In the Tools section of the Agilent Lab Advisor software system screen exit Service & Diagnostics > Maintenance Positions > Change Metering Device: click Next and wait until the metering device is in Home position. 17 Select Back to finish the procedure. 18 Close the doors. 19 Perform a pressure test.
  

• Agilent 1260 Infinity II Preparative Autosampler User Manual 67 Installation of the Optional Sample Cooler 4 Using the Sample Cooler in an Infinity II Sampler Control The Cooler section of the Control dialog box is available when you have a cooler installed. It allows you to switch the cooler on and off manually. Select On to switch on the cooler. Specify the required temperature in the adjacent field. Note that the specified temperature must be at least 5 °C below ambient for proper temperature control. Select Off to switch off the cooler.
  

• Agilent Technologies 1260 Infinity II 158 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Needle Assembly 13 In Lab Advisor use Next to lift the needle slowly into the up position. 14 Turn or flap the wash port to the right. 15 Install the safety cover (1) and fix the screw (2). 16 Select Next to move the needle into the needle seat. 17 Select Back to finish the procedure. 18 Close the doors. 19 Perform a pressure test. 1 2
  

• Agilent Technologies 1260 Infinity II Agilent 1260 Infinity II Preparative Autosampler User Manual 215 Hardware Information 12 Electrical Connections Electrical Connections • The CAN bus is a serial bus with high-speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization. • The ERI/REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shutdown, prepare, and so on. • With the appropriate software, the LAN connector may be used to control th

Similar Devices: OSD-40DP, IMI SENSORS X649A03, F3SG series

• Page 1
  Agilent 1260 Infinity Isocratic Pump and Quaternary Pump User Manual Agilent Technologies…
• Page 2
  Notices Warranty © Agilent Technologies, Inc. 2010 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- 1987). U.S. Government users will receive in any form or by any means (including elec- ment is provided “as is,”…

• Page 3: Table Of Contents

  Contents Contents 1 Introduction Introduction to the Pump Overview of the Hydraulic Path Early Maintenance Feetback Instrument Layout Electrical Connections Interfaces Setting the 8-bit Configuration Switch 2 Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications 3 Installing the Pump Unpacking the Pump Optimizing the Stack Configuration Installing the Pump…

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  Contents 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators User Interfaces Agilent Lab Advisor Software 7 Error Information What Are Error Messages General Error Messages Module Error Messages 8 Test Functions and Calibration Introduction System Pressure Test Leak Rate Test 9 Maintenance…
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  External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer 12 Appendix General Safety Information The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent 1260 IsoPump/QuatPump User Manual…
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  Contents Agilent 1260 IsoPump/QuatPump User Manual…

• Page 7: Introduction

  Electrical Connections Serial Number Information (ALL) Rear View of the Module Interfaces Overview Interfaces Setting the 8-bit Configuration Switch Communication Settings for RS-232C Special Settings This chapter gives an introduction to the module, instrument overview and internal connectors Agilent Technologies…

• Page 8: Introduction To The Pump

  Introduction Introduction to the Pump Introduction to the Pump Introduction to the Quaternary Pump The quaternary pump comprises a solvent cabinet, a vacuum degasser and a four-channel gradient pump. The four-channel gradient pump comprises a high-speed proportioning valve and a pump assembly. It provides gradient generation by low pressure mixing.

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  Introduction Introduction to the Pump Introduction to the Isocratic Pump The isocratic pump has the same operating principle as the quaternary pump but has only one solvent channel, that means the composition cannot be changed during a method because there is no multi-channel gradient valve (MCGV).

• Page 10: Overview Of The Hydraulic Path

  Introduction Overview of the Hydraulic Path Overview of the Hydraulic Path Both the isocratic pump and quaternary pump are based on a two-channel, dual-plunger in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 600 bar.

• Page 11
  Introduction Overview of the Hydraulic Path Hydraulic Path Figure 3 Hydraulic Path of the Isocratic Pump Agilent 1260 IsoPump/QuatPump User Manual…
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  Introduction Overview of the Hydraulic Path Figure 4 Hydraulic Path of the Quaternary Pump How Does the Pump Work? In the quaternary pump, the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve. For the isocratic pump, the solvent bottle is directly connected to the inlet valve.
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  Introduction Overview of the Hydraulic Path limit and leaves it at its top. The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap inbetween. The first piston has a stroke volume in the range of 20 – 100 µL depending on the flow rate.
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  Introduction Overview of the Hydraulic Path When turned on, the pump runs through an initialization procedure to determine the upper dead position of the first piston. The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance.
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  Introduction Overview of the Hydraulic Path Table 2 Quaternary Pump Details Delay volume 800 – 1100 µL, dependent on back pressure Materials in contact with mobile phase MCGV PTFE Pump head SST, gold, sapphire, ceramic Active inlet valve SST, gold, sapphire, ruby, ceramic, PTFE Outlet valve SST, gold, sapphire, ruby Adapter…
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  Introduction Overview of the Hydraulic Path With a compressibility value set the processor calculates a compensation volume, that depends on the back pressure of the system and the selected compressibility. This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston.

• Page 17: Early Maintenance Feetback

  Introduction Early Maintenance Feetback Early Maintenance Feetback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.

• Page 18: Instrument Layout

  Introduction Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.

• Page 19: Electrical Connections

  There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

• Page 20
  Introduction Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing (DE Germany) Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number…

• Page 21: Interfaces

  Introduction Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 3 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump…

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  Introduction Interfaces Table 3 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote G4212A/B DAD G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD G1362A RID G4280A ELSD EXT Contact AUTOZERO Others G1316A/C TCC G1322A DEG G1379B DEG…
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  Introduction Interfaces Overview Interfaces The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for an LAN card (e.g. Agilent G1369A/B LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD).
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  Introduction Interfaces Table 4 RS-232C Connection Table Direction Function Ground Figure 7 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1260 IsoPump/QuatPump User Manual…
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  APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
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  Introduction Interfaces Table 5 Remote Signal Distribution Signal Description Not used POWER ON (H) All modules connected to system are switched on. Receiver is any module relying on operation of others. READY (H) System is ready for next analysis. Receiver is any sequence controller.

• Page 27: Setting The 8-Bit Configuration Switch

  Introduction Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch (On-Board LAN) The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN, e.g.

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  Introduction Setting the 8-bit Configuration Switch Table 6 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW 7 SW 8 Link Configuration Init Mode Selection Auto-negotiation 10 MBit, half-duplex 10 MBit, full-duplex 100 MBit, half-duplex 100 MBit, full-duplex…
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  • for boot/test modes DIPS 1+2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address, serial communication protocol and instrument specific initialization procedures. With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN.
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  Introduction Setting the 8-bit Configuration Switch Table 7 8-bit Configuration Switch (without on-board LAN) Mode Select RS-232C Baudrate Data Parity Bits Reserved Reserved TEST/BOOT RSVD RSVD RSVD The LAN settings are done on the LAN Interface Card G1369A/B. Refer to the N O T E documentation provided with the card.
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  Introduction Setting the 8-bit Configuration Switch Table 9 Baudrate Settings (without on-board LAN) Switches Baud Rate Switches Baud Rate 9600 9600 1200 14400 2400 19200 4800 38400 Table 10 Data Bit Settings (without on-board LAN) Switch 6 Data Word Size 7 Bit Communication 8 Bit Communication Table 11…
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  Introduction Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). The tables include both settings for modules – with on-board LAN and without on-board N O T E LAN. They are identified as LAN and no LAN. Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part).
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  Introduction Setting the 8-bit Configuration Switch Table 13 Forced Cold Start Settings (without on-board LAN) Mode Select TEST/BOOT No LAN TEST/BOOT Agilent 1260 IsoPump/QuatPump User Manual…
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  Introduction Setting the 8-bit Configuration Switch Agilent 1260 IsoPump/QuatPump User Manual…

• Page 35: Site Requirements And Specifications

  Agilent 1260 IsoPump/QuatPump User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies…

• Page 36: Site Requirements

  Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 14 on page 39.

• Page 37
  Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
• Page 38
  Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment. ➔ Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. Bench Space The module dimensions and weight (see…

• Page 39: Physical Specifications

  Site Requirements and Specifications Physical Specifications Physical Specifications Table 14 Physical Specifications Type Specification Comments Weight 11 kg (25 lbs) Dimensions (height × 180 x 345 x 435 mm (7.0 x 13.5 x 17 inches) width × depth) Line voltage 100 –…

• Page 40: Performance Specifications

  Site Requirements and Specifications Performance Specifications Performance Specifications Table 15 Performance Specification Agilent 1260 Infinity Isocratic Pump (G1310B) Type Specification Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Flow range 0.2 –…

• Page 41
  Site Requirements and Specifications Performance Specifications Table 16 Performance Specification Agilent 1260 Infinity Quaternary Pump (G1311B) Type Specification Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Flow range 0.2 –…
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  Site Requirements and Specifications Performance Specifications Agilent 1260 IsoPump/QuatPump User Manual…

• Page 43: Installing The Pump

  Connecting Control Software and/or G4208 A Instant Pilot Flow Connections of the Pump Priming the System Initial Priming Regular Priming Changing Solvents This chapter gives information about the preferred stack setup for your system and the installation of your module. Agilent Technologies…

• Page 44: Unpacking The Pump

  Unpacking the Pump Unpacking the Pump If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. «Defective on arrival» problems C A U T I O N If there are signs of damage, please do not attempt to install the module.

• Page 45
  Accessory Kit (p/n G1311-68755) (see “Accessory Kit G1311-68755” on page 46) Isocratic Pump Checklist Isocratic Pump Checklist Description G1310-64060 Agilent 1260 Infinity Isocratic Pump G1310B 5065-9981 Solvent cabinet, including all plastic parts 9301-1450 Solvent bottle, amber G1311-60003 Bottle-head assembly 5042-2461…

• Page 46: Accessory Kit G1311-68755

  Installing the Pump Unpacking the Pump Accessory Kit G1311-68755 Accessory Kit G1311-68755 Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5988-8453EN Capillary/fitting starter kit brochure 9222-0519…

• Page 47: Optimizing The Stack Configuration

  Installing the Pump Optimizing the Stack Configuration Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.

• Page 48
  Installing the Pump Optimizing the Stack Configuration Figure 10 Recommended Stack Configuration (Front View) Agilent 1260 IsoPump/QuatPump User Manual…
• Page 49
  Installing the Pump Optimizing the Stack Configuration Figure 11 Recommended Stack Configuration (Rear View) Agilent 1260 IsoPump/QuatPump User Manual…

• Page 50: Installing The Pump

  Installing the Pump Installing the Pump Installing the Pump Parts required Description Pump Data System G4208A Instant Pilot Power cord For other cables see text below and “Cable Overview” on page 166. Preparations • Locate bench space. • Provide power connections. •…

• Page 51
  Installing the Pump Installing the Pump 2 Ensure the power switch on the front of the module is OFF (switch stands out). Figure 12 Front of Pump 3 Connect the power cable to the power connector at the rear of the module. Agilent 1260 IsoPump/QuatPump User Manual…
• Page 52
  Installing the Pump Installing the Pump 4 Connect the required interface cables to the quaternary pump, see “Connecting Modules and Control Software” on page 53. 5 Connect all capillaries, solvent tubes and waste tubing (see “Flow Connections of the Pump” on page 56).

• Page 53: Connecting Modules And Control Software

  Connecting Modules and Control Software Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

• Page 54: Connecting A Vacuum Degasser

  Installing the Pump Connecting Modules and Control Software Connecting a Vacuum Degasser The quaternary pump has a built-in degasser. For the isocratic pump, an external degasser N O T E can be used and a pump upgrade to the quaterary pump including a built-in degasser is possible.

• Page 55: Connecting Control Software And/Or G4208 A Instant Pilot

  Installing the Pump Connecting Modules and Control Software Connecting Control Software and/or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN. Usually the detector is producing the most data in the stack, followed by the pump, and it is N O T E therefore highly recommended to use either of these modules for the LAN connection.

• Page 56: Flow Connections Of The Pump

  Installing the Pump Flow Connections of the Pump Flow Connections of the Pump Parts required Description Other modules Parts from accessory kit wrenches 1/4 — 5/16 inch for capillary connections Preparations Pump is installed in the LC system Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks.

• Page 57
  6 Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system. 7 If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack, connect the corrugated waste tube to the waste outlet of the pump leak handling system.
• Page 58
  Installing the Pump Flow Connections of the Pump 9 Prime your system before first use (see “Initial Priming” on page 59). Figure 14 Flow Connections of the Quaternary Pump Agilent 1260 IsoPump/QuatPump User Manual…

• Page 59: Priming The System

  Installing the Pump Priming the System Priming the System Initial Priming When Before a new degasser or new solvent tubing can be used, it is necessary to prime the system. Isopropanol (IPA) is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties.

• Page 60
  Installing the Pump Priming the System When priming the vacuum degasser with a syringe, the solvent is drawn through the N O T E degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 10 minutes at your desired flow rate before starting an analysis.

• Page 61: Regular Priming

  Installing the Pump Priming the System Regular Priming When When the pumping system has been turned off for a certain time (for example, overnight) air will rediffuse into the solvent channel between the vacuum degasser and the pump. Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time.

• Page 62: Changing Solvents

  Installing the Pump Priming the System Changing Solvents When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents are immiscible or one solvent contains a buffer) it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.

• Page 63
  Installing the Pump Priming the System Table 18 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of When switching between Isopropanol the system reverse phase and normal Miscible with almost all phase (both times) solvents After an installation…
• Page 64
  Installing the Pump Priming the System Agilent 1260 IsoPump/QuatPump User Manual…

• Page 65: Using The Pump

  Using the Pump Hints for Successful Use of the Isocratic Pump Prevent Blocking of Solvent Filters Algae Growth in HPLC Systems How to Prevent and/or Reduce the Algae Problem This chapter explains the operational parameters of the module. Agilent Technologies…

• Page 66: Hints For Successful Use Of The Isocratic Pump

  • Always place solvent cabinet with the solvent bottle(s) on top of the pump (or at a higher level). • When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.

• Page 67
  • Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level). • When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump VL it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.

• Page 68: Prevent Blocking Of Solvent Filters

  Using the Pump Prevent Blocking of Solvent Filters Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module. This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7). The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module.

• Page 69: Algae Growth In Hplc Systems

  Agilent 1260 Infinity where helium is not used for degassing (most algae need oxygen and light for growth). The presence of algae in the Agilent 1260 Infinity can cause the following to occur: • PTFE frits, PTFE frit (pack of 5) (p/n 01018-22707), (purge valve assembly) and column filter blockage causing increased system pressure.

• Page 70
  Using the Pump Algae Growth in HPLC Systems frits to block over short-term usage. Please refer to the section “Exchanging the Purge Valve Frit or the Purge Valve” on page 126 in this manual. • Short lifetime of solvent filters (bottle head assembly). A blocked solvent filter in the bottle, especially when only partly blocked, is more difficult to identify and may show up as gradient performance problems, intermittent pressure fluctuations etc.

• Page 71: Optimiziming Performance

  Operational Hints for the Multi Channel Gradient Valve (MCGV) When to use the Seal Wash Option Choosing the Right Pump Seals Optimize the Compressibility Compensation Setting This chapter gives hints on how to optimize the performance or use additional devices. Agilent Technologies…

• Page 72: When To Use A Vacuum Degasser

  • pump was turned off for a length of time (for example, during night) and volatile solvent mixtures are used, or • solvents have been changed. For more information see the Agilent 1260 Infinity Standard Degasser User Manual (p/n G1322-90012). Agilent 1260 IsoPump/QuatPump User Manual…

• Page 73: Operational Hints For The Multi Channel Gradient Valve (Mcgv)

  Optimiziming Performance Operational Hints for the Multi Channel Gradient Valve (MCGV) Operational Hints for the Multi Channel Gradient Valve (MCGV) In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations. However in the mixing point of the gradient valve, at the boundary between the two solvents, micro precipitation is possible.

• Page 74: When To Use The Seal Wash Option

  Optimiziming Performance When to use the Seal Wash Option When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.

• Page 75
  Optimiziming Performance When to use the Seal Wash Option When to Use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.

• Page 76: Choosing The Right Pump Seals

  Optimiziming Performance Choosing the Right Pump Seals Choosing the Right Pump Seals The standard seal for the pump can be used for most applications. However applications that use normal phase solvents (for example, hexane) are not suited for the standard seal and require a different seal when used for a longer time in the pump.

• Page 77: Optimize The Compressibility Compensation Setting

  Optimiziming Performance Optimize the Compressibility Compensation Setting Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 × 10 /bar for the pump. This setting represents an average value. Under normal conditions the default setting reduces the pressure pulsation to values (below 1% of system pressure) that will be sufficient for most applications and for all gradient analyses.

• Page 78
  Optimiziming Performance Optimize the Compressibility Compensation Setting Table 19 Solvent Compressibility Solvent (pure) Compressibility (10-6/bar) Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water Agilent 1260 IsoPump/QuatPump User Manual…

• Page 79: Troubleshooting And Diagnostics

  Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies…

• Page 80: Overview Of The Module’s Indicators And Test Functions

  Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.

• Page 81
  Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump. When a problem with the pump is suspected, use this test to help troubleshoot the pump and its pumping performance.

• Page 82: Status Indicators

  Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. Figure 15 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch.

• Page 83: Module Status Indicator

  Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. •…

• Page 84: User Interfaces

  Troubleshooting and Diagnostics User Interfaces User Interfaces Depending on the user interface, the available tests vary. Some descriptions are only available in the service manual. Table 20 Test functions available vs. user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Leak Rate Test Agilent 1260 IsoPump/QuatPump User Manual…

• Page 85: Agilent Lab Advisor Software

  Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet.

• Page 86
  Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 1260 IsoPump/QuatPump User Manual…

• Page 87: Error Information

  Restart Without Cover Solvent Zero Counter Pressure Above Upper Limit Pressure Below Lower Limit Pressure Signal Missing Missing Pressure Reading Pump Configuration Valve Fuse Inlet-Valve Fuse Valve Failed (MCGV/SSV) Motor-Drive Power Encoder Missing Inlet-Valve Missing Temperature Out of Range Agilent Technologies…

• Page 88
  Error Information Agilent Lab Advisor Software Temperature Limit Exceeded Servo Restart Failed Pump Head Missing Index Limit Index Adjustment Index Missing Stroke Length Initialization Failed Wait Timeout Degasser: cannot read signal Degasser: limit not reached This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

• Page 89: What Are Error Messages

  Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

• Page 90: General Error Messages

  Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and The analysis was completed successfully, source of a not-ready condition.

• Page 91: Remote Timeout

  Error Information General Error Messages Probable cause Suggested actions Check external instruments for a shut-down Shut-down in an external instrument with a condition. remote connection to the system. Check the vacuum degasser for an error The degasser failed to generate sufficient condition.

• Page 92: Synchronization Lost

  Error Information General Error Messages Synchronization Lost During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.

• Page 93: Leak Sensor Open

  Error Information General Error Messages Leak Sensor Open The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits.

• Page 94: Compensation Sensor Open

  Error Information General Error Messages Compensation Sensor Open The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.

• Page 95: Fan Failed

  Error Information General Error Messages Fan Failed The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.

• Page 96: Module Error Messages

  Error Information Module Error Messages Module Error Messages These errors are pump specific. Restart Without Cover The module was restarted with the top cover and foam open. The sensor on the main board detects when the top foam is in place. If the module is restarted with the foam removed, the module switches off within 30 s, and the error message is generated.

• Page 97: Pressure Above Upper Limit

  Error Information Module Error Messages Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit. Probable cause Suggested actions Ensure the upper pressure limit is set to a value Upper pressure limit set too low. suitable for the analysis. Check for blockage in the flowpath.

• Page 98: Pressure Signal Missing

  Error Information Module Error Messages Probable cause Suggested actions Please contact your Agilent service Defective damper. representative. Please contact your Agilent service Defective main board. representative. Pressure Signal Missing The pressure signal of the damper is missing. The pressure signal of the damper must be within a specific voltage range. If the pressure signal is missing, the processor detects a voltage of approximately -120mV across the damper connector.

• Page 99: Pump Configuration

  Error Information Module Error Messages Pump Configuration At switch-on, the quaternary pump has recognized a new pump configuration. The quaternary pump is assigned its configuration at the factory. If the gradient valve is disconnected, and the quaternary pump is rebooted, the error message is generated.

• Page 100: Inlet-Valve Fuse

  Error Information Module Error Messages Inlet-Valve Fuse The active-inlet valve in the module has drawn excessive current causing the inlet-valve electronic fuse to open. Probable cause Suggested actions Restart the module. If the error message Defective active inlet valve. appears again, exchange the active inlet valve. Please contact your Agilent service Defective connection cable (front panel to representative.

• Page 101: Motor-Drive Power

  Error Information Module Error Messages Probable cause Suggested actions Please contact your Agilent service Connection cable (inside instrument) representative. defective. Exchange the gradient valve. Gradient valve defective. Motor-Drive Power The current drawn by the pump motor exceeded the maximum limit. Blockages in the flow path are usually detected by the pressure sensor in the damper, which result in the pump switching off when the upper pressure limit is exceeded.

• Page 102: Encoder Missing

  Error Information Module Error Messages Encoder Missing The optical encoder on the pump motor in the module is missing or defective. The processor checks the presence of the pump encoder connector every 2 seconds. If the connector is not detected by the processor, the error message is generated.

• Page 103: Temperature Out Of Range

  Error Information Module Error Messages Temperature Out of Range The temperature sensor readings in the motor-drive circuit are out of range. The values supplied to the ADC by the hybrid sensors must be between 0.5 V and 4.3 V. If the values are outside this range, the error message is generated. Probable cause Suggested actions Please contact your Agilent service…

• Page 104: Servo Restart Failed

  Error Information Module Error Messages Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting. When the module is switched on, the first step is to switch on the C phase of the variable reluctance motor.

• Page 105: Index Limit

  Error Information Module Error Messages Index Limit The time required by the piston to reach the encoder index position was too short (pump). During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached.

• Page 106: Index Missing

  Error Information Module Error Messages Index Missing The encoder index position in the module was not found during initialization. During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached.

• Page 107: Initialization Failed

  Error Information Module Error Messages Initialization Failed The module failed to initialize successfully within the maximum time window. A maximum time is assigned for the complete pump-initialization cycle. If the time is exceeded before initialization is complete, the error message is generated.

• Page 108: Degasser: Cannot Read Signal

  Error Information Module Error Messages Probable cause Suggested actions Ensure that purge valve is closed. Purge valve open. Ensure pump components are seated correctly. Leak at fittings, purge valve, active inlet If there are still signs of a leak, exchange the valve, outlet valve or piston seals.

• Page 109: Degasser: Limit Not Reached

  Error Information Module Error Messages Degasser: limit not reached This error is thrown, if the degasser does not become ready after 8 min, i.e. is higher than 180 mbar. Probable cause Suggested actions Please contact your Agilent service Liquid in degasser tubing. representative.

• Page 110
  Error Information Module Error Messages Agilent 1260 IsoPump/QuatPump User Manual…

• Page 111: Test Functions And Calibration

  Agilent 1260 IsoPump/QuatPump User Manual Test Functions and Calibration Introduction System Pressure Test System Pressure Test failed Leak Rate Test Leak Rate Test Description This chapter describes the tests for the module. Agilent Technologies…

• Page 112: Introduction

  Test Functions and Calibration Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B.01.03. Other user interfaces may not provide any test or just a few. For details on the use of the interface refer to the interface documentation. Table 21 Interfaces and available test functions Interface…

• Page 113: System Pressure Test

  Test Functions and Calibration System Pressure Test System Pressure Test Description The System Pressure Test is a quick, built-in test designed to demonstrate the pressure-tightness of the system. The test is required, if problems with small leaks are suspected, or after maintenance of flow-path components (e.g., pump seals, injection seal) to prove pressure tightness up to 600 bar.

• Page 114: Leak Rate Test

  Test Functions and Calibration Leak Rate Test Leak Rate Test Leak Rate Test Description The Leak Rate Test is a built-in troubleshooting test designed to demonstrate the leak-tightness of the pump. For running the Leak Rate Test and evaluating test results, please refer to the online help of LabAdvisor.

• Page 115: Maintenance

  Maintenance of a Pump Head with Seal Wash Option Reinstalling the Pump Head Assembly Exchanging the Multi-Channel Gradient Valve (MCGV) Exchanging the Optional Interface Board Exchanging the Active Inlet Valve (AIV) or its Cartridge Replacing the Module’s Firmware This chapter describes the maintenance of the module. Agilent Technologies…

• Page 116: Introduction To Maintenance And Repair

  Maintenance Introduction to Maintenance and Repair Introduction to Maintenance and Repair The module is designed for easy repair. The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack. These repairs are described in “Overview of Maintenance”…

• Page 117: Warnings And Cautions

  Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice.

• Page 118
  Maintenance Warnings and Cautions Safety standards for external equipment C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

• Page 119: Overview Of Maintenance

  Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the pump that can be carried out without opening the main cover. Table 22 Simple Repair Procedures Procedure Typical Frequency Notes “Checking and Cleaning the Solvent If solvent filter is blocked Gradient performance problems, intermittent Filter”…

• Page 120: Cleaning The Module

  Maintenance Cleaning the Module Cleaning the Module The module case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent. Do not use an excessively damp cloth as liquid may drip into the module. Liquid dripping into the electronic compartment of your module.

• Page 121: Checking And Cleaning The Solvent Filter

  Maintenance Checking and Cleaning the Solvent Filter Checking and Cleaning the Solvent Filter Small particles can permanently block the capillaries and valves of the module. C A U T I O N Damage of the module. ➔ Always filter solvents. ➔…

• Page 122: Cleaning The Solvent Filter

  Maintenance Checking and Cleaning the Solvent Filter Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid (35 %) LC grade water Beaker Preparations Remove solvent inlet tube from the adapter at the inlet valve. 1 Remove the blocked solvent filter from the bottle-head assembly and place it in a beaker with concentrated nitric acid (35%) for one hour.

• Page 123: Exchanging The Passive Inlet Valve (Piv)

  Maintenance Exchanging the Passive Inlet Valve (PIV) Exchanging the Passive Inlet Valve (PIV) When If internally leaking (backflow) Tools required • Wrench 14 mm • Pair of Tweezers Parts required Description G1312-60066 Passive inlet valve 1 Remove the front cover. 2 Disconnect the solvent inlet tube from the inlet valve (be aware that solvent may leak out of the tube due to hydrostatic flow).

• Page 124: Exchanging The Outlet Valve

  Maintenance Exchanging the Outlet Valve Exchanging the Outlet Valve When If internally leaking Tools required Wrench 1/4 inch Wrench 14 mm Parts required Description G1312-60067 Outlet valve, complete Preparations • Switch off pump at the main power switch • Remove the front cover Before exchanging the outlet valve you can try to clean it in a sonic bath for 5 –…

• Page 125
  Maintenance Exchanging the Outlet Valve Reinstall the outlet valve and tighten the valve. Reconnect the valve capillary. Agilent 1260 IsoPump/QuatPump User Manual…

• Page 126: Exchanging The Purge Valve Frit Or The Purge Valve

  Maintenance Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Purge Valve Frit or the Purge Valve When • Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of > 10 bar across the frit at a flow rate of 5 mL/min of H 0 with purge valve opened) •…

• Page 127
  Maintenance Exchanging the Purge Valve Frit or the Purge Valve Place a new frit into the purge valve with the orientation Insert the purge valve into the pump head and locate the of the frit as shown above. pump outlet capillary and the waste tube. Reinstall the cap with the gold seal.

• Page 128: Removing The Pump Head Assembly

  Maintenance Removing the Pump Head Assembly Removing the Pump Head Assembly When • Exchanging the seals • Exchanging the pistons • Exchanging seals of the seal wash option Tools required • Wrench 1/4 inch • 4-mm hexagonal key Preparations • Switch off pump at the main power switch and unplug the power cable •…

• Page 129
  Maintenance Removing the Pump Head Assembly Next Steps: Using a 4 mm hexagonal key, stepwise loosen the two pump head screws and remove the pump head from the pump drive. Disconnect the capillary from the outlet valve. Remove the waste tubing and disconnect the solvent tubing from the inlet valve.

• Page 130: Maintenance Of A Pump Head Without Seal Wash Option

  Maintenance Maintenance of a Pump Head Without Seal Wash Option Maintenance of a Pump Head Without Seal Wash Option When In case of maintenance or pump head internal leaks. Tools required • Wrench 1/4 inch • 4-mm hexagonal key Parts required Description 01018-23702 Insert tool…

• Page 131
  Maintenance Maintenance of a Pump Head Without Seal Wash Option Place the pump head on a flat surface. Loosen the lock Remove the support rings from the piston housing and lift screw (two revolutions) and while holding the lower half the housing away from the pistons.
• Page 132
  Maintenance Maintenance of a Pump Head Without Seal Wash Option Check the piston surface and remove any deposits or Using the insert tool carefully remove the seal from the layers. Cleaning can be done with alcohol or tooth paste. pump head. Remove wear retainers, if still present. Replace piston if scratched.
• Page 133
  Maintenance Maintenance of a Pump Head Without Seal Wash Option Next Steps: If a standard seal has been installed, run the seal wear-in procedure, see “Seal Wear-in Procedure” on page 134. For the normal phase seal, the purge valve frit should be replaced, see “Exchanging the Purge Valve Frit or the Purge Valve”…

• Page 134: Seal Wear-In Procedure

  Maintenance Seal Wear-in Procedure Seal Wear-in Procedure Parts required Description 0100-1847 Adapter AIV to solvent inlet tubes 5022-2159 Restriction capillary Seal damage C A U T I O N ➔ This procedure is required for Standard seals (pack of 2) (p/n 5063-6589), but it will damage the PE seals (pack of 2) (p/n 0905-1420).

• Page 135: Maintenance Of A Pump Head With Seal Wash Option

  Maintenance Maintenance of a Pump Head with Seal Wash Option Maintenance of a Pump Head with Seal Wash Option When When maintaining seal wash option Tools required • 4-mm hexagonal key Parts required Description 01018-23702 Insert tool 0905-1175 Wash seal 5062-2484 Gasket, seal wash (pack of 6) Preparations…

• Page 136
  Maintenance Maintenance of a Pump Head with Seal Wash Option Remove the seal holder and the seal wash support rings Using the blade of a flat-blade screwdriver remove the from the piston housing. Remove the seal holder from the seal wash gasket and the secondary seal from the support ring assembly.
• Page 137
  Maintenance Maintenance of a Pump Head with Seal Wash Option Place the support rings on the piston housing (pistons not Insert the pistons and carefully press them into the seal. installed) and snap the pump head and piston housing together. Tighten the lock screw.

• Page 138: Reinstalling The Pump Head Assembly

  Maintenance Reinstalling the Pump Head Assembly Reinstalling the Pump Head Assembly When When reassembling the pump Tools required • 4-mm hexagonal key Parts required Description 79846-65501 Pump head grease Apply a small amount of grease on the back of the Slide the pump head assembly onto the pump drive.

• Page 139
  Maintenance Reinstalling the Pump Head Assembly Using a 4 mm hexagonal key tighten the pump head Reconnect all capillaries, tubes and (if installed) the screws stepwise with increasing torque. active inlet valve cable to its connector. Reinstall the front cover. Agilent 1260 IsoPump/QuatPump User Manual…

• Page 140: Exchanging The Multi-Channel Gradient Valve (Mcgv)

  When using buffers in combination with organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the aequous solutions/buffers to one of the bottom ports and the organic solvent to one of the upper gradient valve ports.

• Page 141
  Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) Disconnect the connecting tube, waste tube and the Press the lower sides of the cover to unclip it. Remove the solvent tubes from the MCGV, unclip them from the tube cover. clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure.
• Page 142
  Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) Install the MCGV cover. Reconnect the waste funnel with Reconnect the tube from the inlet valve to the middle the waste tube holder in the top cover. Insert waste tube position of the MCGV. Connect solvent tubes for channels in the holder in the leak pan and clip tube to the MCGV A-D from the MCGV to the degasser outlets.

• Page 143: Exchanging The Optional Interface Board

  Maintenance Exchanging the Optional Interface Board Exchanging the Optional Interface Board When Board defective Parts required Description G1351-68701 Interface board (BCD) with external contacts and BCD outputs Electronic boards are static sensitive and should be handled with care so as not to C A U T I O N damage them.

• Page 144
  Maintenance Exchanging the Optional Interface Board 6 Reconnect the pump to line power. Figure 17 Exchanging the Interface Board Agilent 1260 IsoPump/QuatPump User Manual…

• Page 145: Exchanging The Active Inlet Valve (Aiv) Or Its Cartridge

  This is a configuration change which is not covered by the specifications for this module. By default, 1260 Infinity pumps do not have an active inlet valve. The first time an AIV shall N O T E be installed, a connector and cable to the main board must be installed by the service and a different connection tube is needed.

• Page 146
  Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head. Figure 18 Active Inlet Valve Assembly 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly.

• Page 147: Replacing The Module’s Firmware

  Maintenance Replacing the Module’s Firmware Replacing the Module’s Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •…

• Page 148
  Maintenance Replacing the Module’s Firmware Agilent 1260 IsoPump/QuatPump User Manual…

• Page 149: Parts For Maintenance

  Pump Head Assembly with Seal Wash Option (600 bar) Outlet Valve Purge Valve Assembly Active Inlet Valve Assembly Accessory Kit G1311-68755 Seal Wash Option Kit Solvent Cabinet Bottle Head Assembly Hydraulic Path This chapter provides information on parts for maintenance. Agilent Technologies…

• Page 150: Pump Head Assembly Without Seal Wash

  Parts for Maintenance Pump Head Assembly Without Seal Wash Pump Head Assembly Without Seal Wash Item p/n Description G1312-60064 Pump Head without Seal Wash 5067-4695 Sapphire piston (default) G1312-60062 Piston housing (incl. spring) G4220-63015 Support Ring without Seal Wash 0905-1503 Piston seal PTFE, carbon filled, black (pack of 2), default 0905-1719 Pump Seal PE, yellow (pack of 2)

• Page 151
  Parts for Maintenance Pump Head Assembly Without Seal Wash Figure 19 Pump Head Assembly without seal wash option Agilent 1260 IsoPump/QuatPump User Manual…

• Page 152: Pump Head Assembly With Seal Wash Option (600 Bar)

  Parts for Maintenance Pump Head Assembly with Seal Wash Option (600 bar) Pump Head Assembly with Seal Wash Option (600 bar) Item p/n Description G1312-60065 Pump Head with Seal Wash 5067-4695 Sapphire piston (default) G1312-60062 Piston housing (incl. spring) G4220-63010 Support Ring (Seal Wash) 0905-1175 Wash seal PTFE, carbon filled…

• Page 153
  Parts for Maintenance Pump Head Assembly with Seal Wash Option (600 bar) Item p/n Description 0515-2118 Screw M5, 60 mm long G1311-60161 Seal wash pump assembly Figure 20 Pump Head with Seal Wash Option Agilent 1260 IsoPump/QuatPump User Manual…

• Page 154: Outlet Valve

  Parts for Maintenance Outlet Valve Outlet Valve Description G1312-60067 Outlet valve, complete Figure 21 Outlet Valve Agilent 1260 IsoPump/QuatPump User Manual…

• Page 155: Purge Valve Assembly

  Parts for Maintenance Purge Valve Assembly Purge Valve Assembly Item p/n Description G1312-60061 Purge valve assembly 01018-22707 PTFE frit (pack of 5) 5067-4728 Seal cap Agilent 1260 IsoPump/QuatPump User Manual…

• Page 156: Active Inlet Valve Assembly

  Parts for Maintenance Active Inlet Valve Assembly Active Inlet Valve Assembly Item p/n Description G1312-60025 Active inlet valve without cartridge G1312-60020 Cartridge for active inlet valve 600bar Figure 22 Active Inlet Valve Assembly Agilent 1260 IsoPump/QuatPump User Manual…

• Page 157: Accessory Kit G1311-68755

  Parts for Maintenance Accessory Kit G1311-68755 Accessory Kit G1311-68755 Accessory Kit G1311-68755 Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5988-8453EN Capillary/fitting starter kit brochure 9222-0519…

• Page 158: Seal Wash Option Kit

  Parts for Maintenance Seal Wash Option Kit Seal Wash Option Kit Description G1311-60161 Seal wash pump assembly 5042-8507 Seal wash pump cartridge (silicone tubing) 5062-2465 Support ring, seal wash 0905-1175 Secondary seal (pre-installed in support rings) 5062-2484 Gasket, seal wash (pack of 6) 5001-3743 Seal holder 0890-1764…

• Page 159: Solvent Cabinet

  Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p/n Description 5065-9981 Solvent cabinet, including all plastic parts 5043-0207 Name plate 1260 5042-8567 Leak pan 9301-1420 Solvent bottle, transparent 9301-1450 Solvent bottle, amber G1311-60003 Bottle-head assembly Agilent 1260 IsoPump/QuatPump User Manual…

• Page 160
  Parts for Maintenance Solvent Cabinet Figure 23 Solvent Cabinet Parts Agilent 1260 IsoPump/QuatPump User Manual…

• Page 161: Bottle Head Assembly

  Parts for Maintenance Bottle Head Assembly Bottle Head Assembly Item p/n Description G1311-60003 Bottle-head assembly 5063-6598 Ferrules with lock ring (10x) 5063-6599 Tube screw (10x) Wire marker 5062-2483 Solvent tubing, 5 m 5062-8517 Inlet filter adapter (pack of 4) 5041-2168 Solvent inlet filter, 20 µm Figure 24 Bottle-Head Assembly Parts…

• Page 162: Hydraulic Path

  Parts for Maintenance Hydraulic Path Hydraulic Path Item p/n Description G1311-67301 Capillary, piston 1 to damper G1311-60003 Bottle-head assembly G1311-67300 Capillary, damper to piston 2 G1312-67305 Outlet capillary, pump to injector device G1329-87300 Outlet capillary, pump to thermostattable autosampler 5062-2461 Waste tube, 5 m (reorder pack) 0100-1847 Adapter AIV to solvent inlet tubes…

• Page 163
  Parts for Maintenance Hydraulic Path Hydraulic Path Item p/n Description G1312-67305 Outlet capillary, pump to injector device G1329-87300 Outlet capillary, pump to thermostattable autosampler G1311-60003 Bottle-head assembly G1322-67300 Kit of 4 solvent tubes for connection degasser to MCGV (Quaternary Pump) including labels G1311-81600 Capillary, piston 1 to damper G1311-81601…
• Page 164
  Parts for Maintenance Hydraulic Path Figure 26 Hydraulic Flow Path of the Quaternary Pump Agilent 1260 IsoPump/QuatPump User Manual…

• Page 165: Identifying Cables

  Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent Technologies…

• Page 166: Cable Overview

  Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators…

• Page 167
  Identifying Cables Cable Overview CAN cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) External Contact Cable…

• Page 168: Analog Cables

  Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name…

• Page 169
  Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog — Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin 3394/6 Pin Agilent Signal Name module Not connected Black Analog -…

• Page 170: Remote Cables

  Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators…

• Page 171
  Identifying Cables Remote Cables Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent Signal Name Active module (TTL) 1 — White Digital ground 2 — Brown Prepare run 3 — Gray Start 4 — Blue Shut down 5 — Pink Not connected…
• Page 172
  Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 Pin Universal Pin Agilent Signal Name Active module (TTL) 1 — White Digital ground 2 — Brown Prepare run 3 — Gray Start 4 — Blue Shut down 5 — Pink Not connected 6 — Yellow Power on…

• Page 173: Bcd Cables

  Identifying Cables BCD Cables BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent Signal Name…

• Page 174
  Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1260 IsoPump/QuatPump User Manual…

• Page 175: Can/Lan Cables

  Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203…

• Page 176: External Contact Cable

  Identifying Cables External Contact Cable External Contact Cable One end of this cable provides a 15-pin plug to be connected to Agilent modules interface board. The other end is for general purpose. Agilent Module Interface Board to general purposes p/n G1103-61611 Color Pin Agilent Signal Name…

• Page 177: Agilent Module To Pc

  Identifying Cables Agilent Module to PC Agilent Module to PC Description G1530-60600 RS-232 cable, 2 m RS232-61600 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It’s also called «Null Modem Cable»…

• Page 178: Agilent 1200 Module To Printer

  Identifying Cables Agilent 1200 Module to Printer Agilent 1200 Module to Printer Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module. Agilent 1260 IsoPump/QuatPump User Manual…

• Page 179: Appendix

  Agilent 1260 IsoPump/QuatPump User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies…

• Page 180: General Safety Information

  Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.

• Page 181
  Appendix General Safety Information Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed.
• Page 182
  Appendix General Safety Information Safety Symbols Table 23 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.

• Page 183: The Waste Electrical And Electronic Equipment Directive

  Appendix The Waste Electrical and Electronic Equipment Directive The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005.

• Page 184: Batteries Information

  Appendix Batteries Information Batteries Information Lithium batteries may not be disposed-off into the domestic waste. Transportation of WA R N I N G discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Danger of explosion if battery is incorrectly replaced. ➔…

• Page 185: Radio Interference

  Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or…

• Page 186: Sound Emission

  Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •…

• Page 187: Solvent Information

  Appendix Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Small particles can permanently block capillaries and valves. Therefore always filter solvents through 0.4 µm filters. •…

• Page 188: Agilent Technologies On Internet

  Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download.

• Page 189
  Index Index AUX output cleaning Communication settings solvent zero counter RS-232C compensation sensor open ball-screw drive compensation sensor short battery configuration composition precision safety information one stack composition range compressibility compensation 15, 40, cable bench space 8-bit configuration switch condensation blockage on-board LAN configuration…
• Page 190
  Index pump error multi channel gradient valve (MCGV) error messages index limit outlet valve 119, compensation sensor open index adjustment passive inlet valve compensation sensor short index missing pistons encoder missing initialization failed pump seals ignition without cover initialization purge valve frit 119, index adjustment purge valve…
• Page 191
  Index missing pressure reading power consideration missing parts power consumption safety class I motor-drive power power cords safety information multi channel gradient valve power switch lithium batteries (MCGV) pressure above upper limit safety pressure below lower limit general information pressure sensor readings standards non-operating altitude pressure pulsation…
• Page 192
  Index special settings variable reluctance motor boot-resident variable stroke volume forced cold start voltage range specification physical status indicator wait timeout stroke volume waste stroke length electrical and electronic stroke volume equipment synchronization lost wear-in system setup and installation procedure optimizing stack configuration WEEE directive weight…
• Page 193
  Index Agilent 1260 IsoPump/QuatPump User Manual…
• Page 194
  In This Book This manual contains technical information about the Agilent 1260 Infinity Isocratic Pump (G1310B) and Quaternary Pump (G1311B). The manual describes the following: • introduction, • site requirements and specifications, • installing the pump, • using the pump, •…